Exposure to cell phone induce oxidative stress in mice preantral follicles during in vitro cultivation: An experimental study|
Koohestani, Najmeh Vafere; Zavareh, Saeed; Lashkarbolouki, Taghi & Azimipour, Fariba
Background: Radiations emitting from mobile phones have been proposed to
affect people’s health, mediated by various mechanisms like induction of oxidative
Objective: This study aims to investigate the effect of cell phone exposure on the
oxidative status of mice preantral follicles (PFs) during in vitro culture.
Materials and Methods: PFs (n = 2580) were isolated mechanically from 16 to 18 day-old
NMRI mice (n = 50) and divided into control and cell phone-exposed groups. PFs were
cultured for 12 days and ovulation was induced using human chorion gonadotropin.
The developmental parameters including size, survival, antral cavity formation,
ovulation and oocyte maturation were assessed. In parallel, enzymatic antioxidants
activities, total antioxidant capacity (TAC), and Malondialdehyde (MDA) levels were
Results: The diameters and the rates of survival, antrum formation, ovulation, and
metaphase II oocytes of exposed PFs to cell phone were significantly lower than
those of the control group (p≤0.001). The PFs exposed to cell phone had significantly
lower superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT)
activity compared with the control group. In the cell phone exposed PFs, the TAC level
was significantly lower (p≤0.001) and MDA levels was significantly higher (p≤0.001),
compared tothe those of control group.
Conclusion: Exposure to cell phone compromised the developmental competence of
mice PFs by increasing oxidative stress.
Ovarian follicle; Cell phone; Oxidative stress; Mice.