Expression of testicular phosphorylated proteins in types 1 and 2 diabetes mellitus in mice: An experimental study|
Sampannang, Apichakan; Arun, Supatcharee; Burawat, Jaturon; Sukhorum, Wannisa & Iamsaard, Sitthichai
Background: Types 1 and 2 diabetes mellitus (DM) are known to be the cause of
sub/infertility. However, the comparisons of potential markers in spermatogenesis and
steroidogenesis in DM males have never been elucidated.
Objective: This study aimed to examine the expressions of tyrosine-phosphorylated
and steroidogenic acute regulatory (StAR) proteins in testis of DM mice.
Materials and Methods: Fifty-six male C57BL/6 mice were divided into four groups
(n = 14/ each): control of MLD-STZ (multiple low doses of streptozotocin), MLD-STZ,
control of HFD-STZ (high-fat diet with STZ), and HFD-STZ. MLD-STZ mice (type 1 DM)
were intraperitoneally (i.p.) injected with STZ at 40 mg/kg BW for five days. HFD-STZ
mice (type 2 DM) received an HFD for 14 days and i.p.-induced by STZ at 85 mg/kg BW
and fed with HFD. At the end of the experiment (days 36 and 72), the expressions of
phosphorylated proteins and StAR were examined.
Results: Tyrosine phosphorylated proteins were localized in late spermatids, luminal
fluid, and Leydig cells. The intensities of phosphorylated 110, 85, 72, 60, and 55 kDas
were lower in the 36 day-DM mice. Although such intensities were present in both
groups, only 85 kDa in the MLD-STZ mice was higher in HFD mice at 72 days. StAR
expressions in both groups were decreased than that of the controls.
Conclusion: Decreased expressions of StAR and tyrosine-phosphorylated proteins
may be directly involved in low testosterone levels and impaired spermatogenesis.
These findings support the notion that both DM types play a role in male
Diabetes mellitus; Testicular phosphorylated protein; Steroidogenic acute regulatory protein; Streptozotocin; Mice.