International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 17, No. 7, 2019, pp. 493-502
Bioline Code: rm19053
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 17, No. 7, 2019, pp. 493-502
© Copyright 2019 - Seddigheh Sheikh Hosseini et al.
The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats|
Hosseini, Seddigheh Sheikh; Gol, Ali & Khaleghi, Moje
Background: Oxidative stress plays an important role in the development of diabetic
Objective: This study evaluated the impact of pre- and post-treatment with Lactobacillus
acidophilus ATCC 4356 on the oxidant and anti-oxidant factors of testis and
epididymis in streptozotocin-induced diabetic rats.
Materials and Methods: Thirty male Wistar rats (10 wk old) weighing 220-230 g. were
divided into five groups (n = 6/ each): 1- normal group, 2- normal lactobacillus group,
3- diabetic group, 4- diabetic + lactobacillus before (DLB) group, and 5- diabetic +
lactobacillus after (DLA) group. The normal and diabetic groups received daily 1 mL
normal saline for 6 wk. Normal lactobacillus group received daily L. acidophilus for 6
wk. Group DLB received daily L. acidophilus for 2 wk before diabetes and for 4 wk after
diabetes. Group DLA received daily 1 mL normal saline for 2 wk before diabetes and L.
acidophilus for 4 wk after diabetes. The dose of L. acidophilus was 1 × 109 CFU/mL.
Results: The administration of L. acidophilus worsened blood glucose level and
reduced the levels of Malondialdehyde (p ≤ 0.0001) and Hydrogen peroxide (p ≤
0.0001) and, Catalase and Glutathione peroxidase activity increased in the testis. In
epididymis, Glutathione peroxidase and Catalase (p = 0.013) activity increased and
Hydrogen peroxide concentration reduced, while Malondialdehyde concentration did
not show any changes compared to the diabetic rats. Also, there was no significant
difference between DLB and DLA groups, in these markers.
Conclusion: Data obtained suggests that L. acidophilus has anti-oxidant effects on the
testis and sometime in the epididymis in diabetic rats.
Diabetes mellitus; Oxidative stress; Rats; Reproduction; Lactobacillus acidophilus.
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