International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 17, No. 7, 2019, pp. 473-480
Bioline Code: rm19050
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 17, No. 7, 2019, pp. 473-480
© Copyright 2019 - Leila Keshavarz et al.
The association of Q472H variant in the KDR gene with recurrent pregnancy loss in Southern Iran: A case-control study|
Keshavarz, Leila & Yavarian, Majid
Background: Recurrent spontaneous abortion (RSA) often remains unclear and can
be burden for the patient and time consuming for clinician. RSA may initiates from a
genetic or non-genetic factors. It is well known that the quality of placental circulation
is critical for implantation and embryo development. Because of angiogenic effects
of VEGF–KDR pathway on placenta, the genes involved in this pathway (the KDR or
VEGFR genes) are thought to be linked with RSA.
Objective: The aim of this study was to investigate the relationship between Gln472His
(A/T) polymorphism of the KDR gene with RSAs in southern Iran.
Materials and Methods: In this case-control study, 50 aborted embryonic tissue
obtained from fetuses and 50 umbilical cord blood of newborn babies were studied.
Fetal sample from mothers with history of at least two consecutive miscarriages and
controls from mothers who had at least one full-term infants born were taken. Genomic
DNA was extracted by using PureLink genomic DNA kit (Life Technologies, CA). The
Rotor-Gene Q real-Time PCR machine and High-resolution melting curve analysis
(HRM) technique were used for genotyping.
Results: Based on the AA genotype as reference, it is shown that the T allele (OR =
2.447, 95% CI = 1.095–5.468, p = 0.029) as well as AT heterozygote genotype was
significantly associated with an increased risk of miscarriage (OR = 2.824, 95% CI =
1.210-6.673, p = 0.016).
Conclusion: A positive correlation between Q472H polymorphism of the KDR gene
and RSA may be the cause in southern Iran.
Abortion; VEGFR 2; Polymorphism.
Alternative site location: http://www.ijrm.ir