International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 17, No. 6, 2019, pp. 405-412
Bioline Code: rm19043
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 17, No. 6, 2019, pp. 405-412
© Copyright 2019 - Parvin Dorfeshan et al.
Expression of miR-302 in human embryo derived from in-vitro matured oocyte|
Dorfeshan, Parvin; Novin, Marefat Ghaffari; Salehi, Mohammad & Farifteh, Fatane
Background: The expression of miR-302 over the period of early embryogenesis could
possibly regulate the maternal transcript clearance. Zygotic transcription activation is
mostly related to maternal messages degradation.
Objective: In this study, the effects of in-vitro maturation technique (IVM) on the
expression of miR-302 in human embryo produced from immature and mature human
oocytes (matured in vitro and in vivo, before sperm exposure) obtained from females
under gonadotrophin therapy were evaluated for assisted reproduction.
Materials and Methods: Immature oocytes were cultured in vitro. The injection of
oocytes-producing polar bodies was given using fresh sperm. Then, the embryo quality
score was assessed in the IVM group compared with the control group. In both the
groups, embryos with normal morphology were included in the molecular study. Only
one blastomere was removed from three-day embryos and then the embryos were
frozen. The expression of miR-302 in embryos was measured through quantitative real-time
polymerase chain reaction.
Results: Our data showed a significant reduction of miR-302 expression in the IVM
group vs. the control group (p = 0.02). The embryo quality score showed a significant
difference between the two groups (p = 0.01).
Conclusion: The present study demonstrated that the IVM process had a negative
effect on the expression level of miR-302 in human pre-implantation embryos.
Considering the major role of expression miR-302, a reduced potential in miR-302
expression could be related to a decrease in the early embryonic development.
miR-302; Embryonic development; Ovarian stimulation; In-vitro maturation; Intracytoplasmic sperm injection.
Alternative site location: http://www.ijrm.ir