Diabetes mellitus increased integrins gene expression in rat endometrium at the time of embryo implantation|
Bakhteyari, Abbas; Zarrin, Yasaman; Nikpour, Parvaneh; Hosseiny, Zeinab Sadat; Mostafavi, Fatemah Sadat; Eskandari, Nahid; Matinfar, Mohammad & Aboutorabi, Roshanak
Background: Diabetes mellitus deeply changes the genes expression of integrin (Itg)
subunits in several cells and tissues such as monocytes, arterial endothelium, kidney
glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate
of successful assisted as well as non-assisted pregnancy. Endometrium undergoes
thorough changes in normal menstrual cycle and the question is: What happens in
the endometrium under diabetic condition?
Objective: The aim of the current study was to investigate the endometrial gene
expression of α3, α4, αv, Itg β1 and β3 subunits in diabetic rat models at the time
of embryo implantation.
Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control
group, diabetic group, pioglitazone-treated group, and metformin-treated group. Real-time
PCR was performed to determine changes in the expression of Itg α3, α4, αv, β1,
and β3 genes in rat’s endometrium.
Results: The expression of all Itg subunits increased significantly in diabetic rats’
endometrium compared with control group. Treatment with pioglitazone significantly
reduced the level of Itg subunits gene expression compared with diabetic rats. While
metformin had a different effect on α3 and α4 and elevated these two subunits gene
Conclusion: Diabetes mellitus significantly increased the expression of studied Itg
subunits, therefore untreated diabetes could be potentially assumed as one of the
preliminary elements in embryo implantation failure.
Diabetes mellitus; Embryo implantation; Integrins; Endometrium.