International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 17, No. 6, 2019, pp. 385-394
Bioline Code: rm19041
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 17, No. 6, 2019, pp. 385-394
© Copyright 2019 - Behpour Yousefi et al.
Clomiphene citrate impairs the endometrial CD98 expression in ovariectomized and non-ovariectomized rats: Role of HCG|
Yousefi, Behpour & Rahbar, Elnaz
Background: Clomiphene citrate (CC) is one of the widely used drugs as an ovulation
stimulant, but its adverse effects on the endometrium results in lowering down the
pregnancy rate. Endometrium CD98 is also important in the process of implantation.
Objective: To evaluate the immunohistochemistry expression levels of endometrial
CD98 following injection of CC with and without Human chorionic gonadotropin (HCG)
in ovariectomized and non-ovariectomized rats.
Materials and Methods: Seventy two (12-14 wk old) female Wistar rats were randomly
divided into two groups (n = 36): (a) ovariectomized and (b) non-ovariectomized. Each
group was further divided into six subgroups (n = 6/each): (1) CC 10 mg/kg, (2) CC 20
mg/kg, (3) HCG, (4) CC 10 mg/kg with HCG, (5) CC 20 mg/kg with HCG, and (6) control.
The experimental subgroups received a single dose of CC (daily, five days) and HCG
(after the last injection of CC) alone or in combination. Immunohistochemistry staining
was performed on paraffin-embedded endometrial tissues to evaluate the expression
levels of CD98.
Result: Animals undergoing ovariectomy presented a significantly lower expression
level of endometrial CD98 (p < 0.001) when compared with non-ovariectomized in
the same condition that groups were subdivided. There was also a dose-dependent
reduction (p < 0.001) in the expression of CD98 in non-ovariectomized subgroups when
compared with control group. In addition, injection of HCG following treatment with CC
improved its expression.
Conclusion: It was concluded that CC impairs CD98 expression in endometrium and
this impairment is intensified with the removal of the ovary. Also, an injection of HCG
following treatment with CC can slightly improve the expression of CD98.
Endometrium; Clomiphene citrate; Human chorionic gonadotropin; CD98.
Alternative site location: http://www.ijrm.ir