International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 17, No. 5, 2019, pp. 337-348
Bioline Code: rm19036
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 17, No. 5, 2019, pp. 337-348
© Copyright 2019 - Hoda Bagherpour et al.
Protective effect of green tea extract on the deltamethrin-induced toxicity in mice testis: An experimental study|
Bagherpour, Hoda; Malekshah, Abbasali Karimpour; Amiri, Fereshteh Talebpour & Azadbakht, Mohammad
Background: Deltamethrin (DM) is one of the environmental factors that can have
destructive effects on the male fertility. Green tea (GT) as a medicinal herb, has
Objective: The present study investigated the protective role of GT extract in improving
the harmful effects of DM on the testis.
Materials and Methods: In this experimental study, 35 adult male mice (25–30 gr) were
divided in to five groups (n = 7/each). The control group received only normal saline.
Sham received 0.2 ml corn oil. Green tea group received only GT of 150 mg/kg. bw;
deltamethrin group received the DM at a dose of 0.6 mg/kg. bw; GT + DM received both
GT and DM. The effect of GT was assessed by measuring oxidative stress markers,
sperm parameters, histological and immunohistochemical analysis.
Results: The results showed that the count and motility of spermatozoa, testosterone,
and Malondialdehyde significantly decreased (p < 0.001) and the abnormal spermatozoa
increased (p < 0.001) in DM group compared to control group. Moreover, enhanced
caspase-3expression and apoptosis were observed in DM-treated mice compared to
control group. Histologically, DM with a degenerative effect on testicular tissue reduced
the spermatogenesis progenitor cells. The epithelial height and the diameter of the
seminiferous tubules were also reduced in the DM group. Treatment with GT in the
DM-treated mice significantly improved these changes.
Conclusion: With these findings, it was concluded that the GT treatment with
antioxidant activity and anti-apoptotic property could protect the testicular injury
induced by DM.
Deltamethrin; Green tea; Oxidative stress; Testis; Caspase-3.
Alternative site location: http://www.ijrm.ir