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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 17, No. 5, 2019, pp. 337-348
Bioline Code: rm19036
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Reproductive BioMedicine, Vol. 17, No. 5, 2019, pp. 337-348

 en Protective effect of green tea extract on the deltamethrin-induced toxicity in mice testis: An experimental study
Bagherpour, Hoda; Malekshah, Abbasali Karimpour; Amiri, Fereshteh Talebpour & Azadbakht, Mohammad

Abstract

Background: Deltamethrin (DM) is one of the environmental factors that can have destructive effects on the male fertility. Green tea (GT) as a medicinal herb, has antioxidant property.
Objective: The present study investigated the protective role of GT extract in improving the harmful effects of DM on the testis.
Materials and Methods: In this experimental study, 35 adult male mice (25–30 gr) were divided in to five groups (n = 7/each). The control group received only normal saline. Sham received 0.2 ml corn oil. Green tea group received only GT of 150 mg/kg. bw; deltamethrin group received the DM at a dose of 0.6 mg/kg. bw; GT + DM received both GT and DM. The effect of GT was assessed by measuring oxidative stress markers, sperm parameters, histological and immunohistochemical analysis.
Results: The results showed that the count and motility of spermatozoa, testosterone, and Malondialdehyde significantly decreased (p < 0.001) and the abnormal spermatozoa increased (p < 0.001) in DM group compared to control group. Moreover, enhanced caspase-3expression and apoptosis were observed in DM-treated mice compared to control group. Histologically, DM with a degenerative effect on testicular tissue reduced the spermatogenesis progenitor cells. The epithelial height and the diameter of the seminiferous tubules were also reduced in the DM group. Treatment with GT in the DM-treated mice significantly improved these changes.
Conclusion: With these findings, it was concluded that the GT treatment with antioxidant activity and anti-apoptotic property could protect the testicular injury induced by DM.

Keywords
Deltamethrin; Green tea; Oxidative stress; Testis; Caspase-3.

 
© Copyright 2019 - Hoda Bagherpour et al.
Alternative site location: http://www.ijrm.ir

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