International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 17, No. 4, 2019, pp. 261-270
Bioline Code: rm19028
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 17, No. 4, 2019, pp. 261-270
© Copyright 2019 - Fatemeh Rahimi Anbarkeh et al.
Effects of monosodium glutamate on apoptosis of germ cells in testicular tissue of adult rat: An experimental study|
Anbarkeh, Fatemeh Rahimi; Baradaran, Raheleh; Ghandy, Nasibeh; Jalali, Mehdi; Nikravesh, Mohammad Reza & Soukhtanloo, Mohammad
Background: Monosodium glutamate (MSG) is used as a flavoring and food seasoning.
Some studies have reported the oxidative effects of using this substance on various
Objective: This study has investigated the effects of MSG and the protective effect of
vitamin C (vit C) on apoptosis of testicular germ cells and biochemical factors.
Materials and Methods: In this experimental study, 24 adult male Wistar rats were
randomly divided into four groups: control (received distilled water), vit C group (150
mg/kg), experimental group 1 (MSG 3 gr/kg), experimental group 2 (MSG 3 gr/kg + vit
C 150 mg/kg). The rats were gavaged for 30 days, and then were sacrificed, the right
testis was isolated for biochemical examinations for the glutathione, malondialdehyde,
and left testis used in histological experiments. Tunnel staining was used to determine
the number of apoptotic cells.
Results: The results showed that apoptotic cells in the MSG group had a significant
increase compared to the control group (P = 0.001), but the number of these cells in
the MSG co-administered with vit C and vit C groups were significantly lower than the
MSG group. Germinal epithelial thickness also decreased in MSG group compared to
the control group.
Conclusion: MSG can lead to increase apoptotic changes in the germinal epithelial of
the testicle, and vit C as an antioxidant can modify the pathological and biochemical
changes induced by MSG.
Apoptosis; Monosodium glutamate; Rat; Testis; Vitamin C.
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