Differential expression of progesterone receptor isoforms related to PGR +331g/a polymorphism in endometriosis: A case-control study|
Mousazadeh, Sepideh; Ghaheri, Azadeh; Shahhoseini, Maryam; Aflatoonian, Reza & Afsharian, Parvaneh
Background: Endometriosis are defined as a progesterone-resistance disease. Two
progesterone receptor (PR) isoforms, namely PR-A and PR-B, mediate the special
effects of progesterone. One of the most effective polymorphism in the promoter region
of PGR is the +331G/A.
Objective: The differential expression level of PR isoforms due to +331G/A polymorphism
may be able to influence the function of progesterone and reduce the
susceptibility of endometriosis.
Materials and Methods: This analytic, case-control study was carried out at Royan
Institute, Tehran, Iran. Whole-blood samples were collected from 98 infertile women
undergoing laparoscopy for endometriosis and 102 healthy fertile women. After DNA
extraction, genotype frequencies were determined by polymerase chain reaction-restriction
fragment length polymorphism. Then, RNA was extracted from the selected
eutopic tissue samples of endometriosis patients. Analysis of PR-A and PR-B mRNA
expressions were performed using Real-time polymerase chain reaction.
Results: The frequency distribution of GG, GA genotypes in +331G/A polymorphism
was 98.04%, 1.96% in the patients and 97.96%, 2.04% in the control groups, respectively
(p = 0.968). Although our data did not show any significant association with +331G/A
in the patient and control groups, we were able to demonstrate significantly higher
expression level of PR-B and no significant lower expression level of PR-A isoforms in
patients by favoring GA to GG genotypes (p = 0.017, p = 0.731, respectively).
Conclusion: Our findings show that patients with GA genotypes had a higher
expression level of PR-B compared to patients with GG genotypes.
Endometriosis; Progesterone receptor A; Progesterone receptor B; rs10895068.