Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study|
Mohsenzadeh, Mehdi; Tabibnejad, Nasim; Vatanparast, Mahboubeh; Anbari, Fatemeh; Khalili, Mohammad Ali & Karimi-Zarchi, Mojgan
Background: In vitro maturation (IVM) of immature oocytes retrieved from ovarian
tissue has been considered as a valuable approach for fertility preservation in
Objective: To evaluate the efficacy of vitrification on oocyte maturation, survival rates,
as well as the subcellular oocyte quality post IVM.
Materials and Methods: The ovarian cortexes from 19 women with cervix and uterine
malignancy aged 21–39 yr were collected. Cumulus-oocyte complexes were aspirated
from all visible antral follicles. 102 immature oocytes were collected, and 43 oocytes
were detected appropriately for IVM (control group). Also, 59 immature oocytes were
vitrified, then matured in vitro (IVM) in two groups: with Growth/differentiation factor
9 (GDF9) (group 1) and without GDF9 (group 2) supplementation. Rates of oocytes
viability, maturation, and survival along with meiotic spindle visualization and zona
pellucida birefringence were assessed with Polyscope.
Results: The rate of maturation was significantly higher in controls (55.8%) compared
to the other groups. Maturation rate was 23.3% in oocytes cultured in IVM medium
enriched with GDF9, and 27.6% in those cultured in IVM medium lacking GDF9 (p
= 0.86). Also, the meiotic spindle was present in 74.4% of control oocytes which
was significantly higher than the other groups. The proportion of high zona pellucida
birefringence was higher in the controls when compared with group 1 (51.2% vs. 23.3%,
respectively, p = 0.04).
Conclusion: Vitrification had a detrimental effect on oocyte maturation, viability as well
as the subcellular quality of the oocytes after IVM in cancerous women.
IVM; Vitrification; Fertility preservation; Maturation rate; Meiotic spindle; Zona pellucida birefringence.