Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic Mice|
Khorramabadi, Khadijeh Mirzaei; Talebi, Ali Reza; Sarcheshmeh, Abolghasem Abbasi & Mirjalili, Aghdas
Background: Generation of free radicals and oxidative stress are a major contributor
to diabetes. These factors lead to the development of diabetic testicles disorders.
Objective: In this study, the protective effect of vitamin E on functional disorders
associated with diabetes induced oxidative stress in male reproductive systems has
Materials and Methods: Thirty-three adult male Mice were divided into control,
diabetic, and untreated diabetic groups. Streptozotocin was used to induce diabetes.
In the treated group, vitamin E was given to the Mice intraperitoneally for 30 days.
Then, animals were anesthetized and sacrificed. Animal testicles were isolated and
homogenized in phosphate buffer and used for measuring sperm count, motility and
survival of sperm, MDA concentration and antioxidant capacity (TAC). Apoptosis was
also performed with the TUNEL test.
Results: The results of reduction (12.03±98.11) TAC, MDA concentration (–28.5±2.58),
sperm motility (unstable sperma= 86.4±7.48), sperm count (171.51), Sperm morphology
(natural morphology= 49.69±31.93) and abnormal morphology (9.77±49.7)
with increased oxidative damage. These changes were statistically significant in
comparison with the control group for all variables other than MDA (p= 0.05). Treatment
of vitamin E diabetic Mice improved the ability of antioxidants to prevent oxidative
damage in the testicles, restore the sperm movement, and increase the number of
normal sperm as well as TAC. The level of apoptosis in the treated group has decreased
compared to the untreated group.
Conclusion: Vitamin E protects the reproductive system against diabetes mellitus.
Therefore, it was concluded that vitamin E may be a suitable agent for protecting the
sperm and testicular parameters against undesirable effects of diabetes.
Case-control study; Vitamin E; Diabetes treatment; Diabetic Syrian mice; Male reproductive dysfunction.