International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 17, No. 1, 2019, pp. 11-22
Bioline Code: rm19002
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 17, No. 1, 2019, pp. 11-22
© Copyright 2019 - Samira Rajaei et al.
Antioxidant effect of genistein on ovarian tissue morphology, oxidant and antioxidant activity in rats with induced polycystic ovary syndrome|
Rajaei, Samira; Alihemmati, Alireza & Abedelahi, Ali
Background: Oxidative stress is the most frequent cause of female infertility disorders
including polycystic ovary syndrome (PCOS). Genistein as a major component of
soybean isoflavone scavenges free radicals by antioxidant activities.
Objective: The present study examines the antioxidant effects of genistein on ovarian
tissue following experimental PCOS in rats.
Materials and Methods: Twenty female Wistar rat were randomly divided into the
following groups (n=5 each group): (I) control group (no treatment); (II) induced PCOS
(injection of estradiol valerate); (III) genistein-treated non-PCOS (received genistein);
and (IV) genistein-treated PCOS groups. The weight of rats were measured and the
blood samples collected and centrifuged. The oxidant and antioxidant activity of
plasma and ovaries were measured. All rats were sacrificed under anesthesia, and
ovaries were collected and weighted. Histological examination and follicular quality
were assessed by staining.
Results: In histological observation, the induced PCOS rats displayed more number
of atretic follicles and the follicular quality in genistein-treated rats was similar to the
control groups. The plasma and ovaries malondialdehyde levels significantly increased
in PCOS rats (p < 0.001), while the total antioxidant capacity levels, glutathione
peroxidase, and superoxide dismutase activities significantly decreased (p < 0.001).
The plasma and ovary malondialdehyde levels significantly decreased in PCOS rats
that were treated with genistein (p < 0.001) and the total antioxidant capacity (p < 0.05),
glutathione peroxidase, and superoxide dismutase activities significantly increased (p
Conclusion: Treatment with genistein preserved follicular quality by increasing
antioxidant activities and scavenging oxidant levels in PCOS rats.
Genistein; Antioxidant; Ovary; Polycystic; Follicle; Rats.
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