International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 16, No. 11, 2018, pp. 711-718
Bioline Code: rm18087
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 16, No. 11, 2018, pp. 711-718
© Copyright 2018 - International Journal of Reproductive BioMedicine
Cyclophosphamide-induced testicular toxicity ameliorate by American ginseng treatment: An experimental study|
Hosseini, Akram; Zare, Samad; Borzouei, Zahra & Pakdel, Firouz Ghaderi
Background: Cyclophosphamide (CP) is one of the most invasive
chemotherapeutic agents, which used commonly despite of its wide spectrum
toxicity. Clinical evidence showed toxic side effects of CP in multiple organ
Objective: The objective of the present study was to evaluate the effects of
American ginseng on CP-induced testicular toxicity in rats.
Materials and Methods: Adult male Wistar rats (220±30 gr) were randomly
divided into four groups (n=7 in each). Group 1 as control received normal saline by
gavage, group 2 received CP (6.1 mg/kg/day, i.p.) for a period of 50 days. Group 3
received American ginseng (500 mg/kg/day) by gavage. Group 4 received American
ginseng (500 mg/kg/day) 1h prior to the administration of CP in the equal dose of
group 2. The animals scarified one day after the last treatment and the effects of
American ginseng on the sperm vital parameters, testicular functions, biochemical
factors, and structural malformations evaluated.
Results: Serum testosterone concentration was significantly decreased whereas the
level of malondialdehyde and DNA damage were significantly increased in animals
of CP group (p<0.01). Co-administration of American ginseng reversed these
parameters and improved recovery in CP+ginseng group. In addition, seminiferous
tubules of testis severely damaged in the CP group but ginseng improved histologic
changes in CP+ginseng group.
Conclusion: The findings confirmed the protective effects of American ginseng on
toxicity induced by CP in the reproductive system of male rats.
Cyclophosphamide; American ginseng; Oxidative stress; DNA damage.
Alternative site location: http://www.ijrm.ir