The effects of vitamin C and menthone on acyclovir induced DNA damage in rat spermatozoa: An experimental study|
Toghiani, Shima; Roudbari, Nasim Hayati; Dashti, Gholam Reza & Rouzbehani, Shahla
Background: Acyclovir (ACV) is known to be toxic to gonads, inducing apoptosis
in the reproductive system. The beneficial effects of vitamin C (Vit C) and
menthone, both as antioxidant agents on various organs has been reported.
Objective: This study evaluated the potential role of the Vit C and menthone on the
DNA damage in rat spermatozoa induced by the ACV.
Materials and Methods: In this experimental study, adult male albino Wistar rats
with average weight of 250±10 gr, were divided into six groups (n=18/each), as:
ACV (15 mg/kg/day), ACV+Vit C (20 mg/kg/day), ACV+ menthone (100 μl/d),
ACV+ menthone (250 μl/d), ACV+ menthone (400 μl/day) and control group
without any treatment. At the end of experiment, the animals were sacrificed and
sperm samples were collected and isolated in phosphate-buffered saline and
examined by TUNEL staining process. The percentage of TUNEL positive
spermatozoa was evaluated by fluorescence microscopy. Each experiment was
performed in three repeats.
Results: Male rats exposed to ACV had significant increase in DNA damages in
comparison to other groups. The percentage of TUNEL positive sperm cells was
90.83 (p<0.001) in ACV group. The protective role of both antioxidants used in high
dose, compensate the adverse effects of the ACV. The results showed that the
percentage of apoptotic sperm in the ACV+Vit C group was 16.38 (p<0.001) and in
the ACV+ menthone (400 μl/d) group was 16.05 (p<0.001).
Conclusion: The present results showed that Vit C and menthone at higher dose
have a good compensatory effect with significant reduction in DNA damages in
sperm cells by reversing the adverse effect of ACV on the reproductive system in
Vitamin C; Menthone; Acyclovir; DNA damage; Rat.