International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 16, No. 8, 2018, pp. 501-506
Bioline Code: rm18060
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 16, No. 8, 2018, pp. 501-506
© Copyright 2018 - International Journal of Reproductive BioMedicine
The relationship between the expression levels of miR-135a and HOXA10 gene in the eutopic and ectopic endometrium|
Mirabutalebi, Seyed Hamidreza; Karami, Noorodin; Montazeri, Fatemeh; Fesahat, Farzaneh; Sheikhha, Mohammad Hasan; Hajimaqsoodi, Elnaz; Zarchi, Mojgan Karimi & Kalantar, Seyed Mehdi
Background: The study of microRNA expression can be effective in the diagnosing
and treating different diseases. miR-135a is one of the most important micro-ribonucleic
acids involved in endometriosis. Among the genes that become the
target of the miR-135a and are subjected to changes in the endometrium of patients
with endometriosis is HOXA10 gene which is expressed in the endometrium in
response to steroid hormones.
Objective: The aim of this study was to evaluate the expression of miR-135a and its
relationship with the level of HOXA10 gene expression in both endometrial ectopic
and eutopic tissues in patients with endometriosis compared to the control samples.
Materials and Methods: In this prospective case-control study, both case-eutopic
and case-ectopic tissue samples were obtained from 17 women with endometriosis
and the eutopic endometrial tissue was sampled from 17 women with normal
endometrium as the control group. The gene's expression of miR-135a and HOXA10
were investigated using quantitative reverse transcription PCR (q-RT PCR).
Results: A significant decrease in the expression of HOXA10 gene was detected in
case-eutopic during the luteal phase compared to the control samples (p=0.001),
while in the case-ectopic, the expression of this gene was increased (p=0.681)
compared to the control samples. In addition, the expression miR-135a in the luteal
phase showed a remarkable increase in the case-eutopic endometrial tissue
(p=0.026) as well as a significant decrease in the case-ectopic endometrial tissue
compared to the control samples (p=0.008).
Conclusion: Considering the inverse relations between the over-expression of miR-135a and the reduction of HOXA10, it seems that miR-135a may be applied as an
endometrial diagnostic and therapeutic biomarker.
Endometriosis; Gene expression; Micro-ribonucleic acid; HOXA10; miR-135a.
Alternative site location: http://www.ijrm.ir