International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 16, No. 7, 2018, pp. 469-474
Bioline Code: rm18056
Full paper language: English
Document type: Short Communication
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 16, No. 7, 2018, pp. 469-474
© Copyright 2018 - International Journal of Reproductive BioMedicine
Assessing the prevalence of Staphylococcus aureus in infertile male patients in Tabriz, northwest Iran|
Esmailkhani, Aylin; Akhi, Mohammad Taghi; Sadeghi, Javid; Niknafs, Behrooz; Bialvaei, Abed Zahedi; Farzadi, Laya & Safadel, Nooshafarin
Background: Staphylococcus aureus is an infrequent, but one of the most
successful bacteria that associated with infertility and are able to spermatozoa
immobilization and agglutination.
Objective: The aim of present study was to determine the frequency of S. aureus in
semen obtained from infertile male patients in northwest Iran.
Materials and Methods: Seminal fluids of 100 infertile men were evaluated.
Standard semen examination was done according to World Health Organization
guidelines. After isolation and identification of S. aureus isolates according to
reference methods, determination of susceptibility against important antibiotics and
polymerase chain reaction were performed to identify mecA and tst genes.
Results: Data obtained from the present study shows that 16% of infertile male
patients were colonized by S. aureus. Ten (62.5%) of the individuals had abnormal
seminal fluid sperm motility and morphology and three (18.8%) of them had an
abnormal seminal fluid density, whereas after washing with albumin-saline declined
to 5 (31.3%), 4 (25%) and 1 (6.3%), respectively. The antibiogram results showed
that, except penicillin, other antibiotics have high activity on isolates. Regarding
polymerase chain reaction results, mecA sequences were detected in 3 (18.7%)
strains, whilst the tst gene encoding TSST-1 was not detected in any of clinical
Conclusion: It would appear that the S. aureus may be an additional negative factor
worsening sperm quality and affecting male fertility. Therefore, it demands that all
the patients attending in infertility treatment facilities be investigated thoroughly.
Infertility; Staphylococcus aureus; Seminal fluid; MecA.
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