Erythrocyte and spermatozoa glucose-6-phosphate dehydrogenase activity in merino rams: An experimental study|
Gurel, Hüseyin; Baspinar, Nuri; Akalin, Pınar Peker; Altunok, Vahdettin & Kazak, Filiz
Background: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of
the pentose phosphate metabolic pathway that supplies reducing agents by
maintaining the level of reduced nicotinamide adenine dinucleotide phosphate.
Objective: It was aimed to determine the activity of erythrocyte and spermatozoa
G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find
out the relation of these parameters with sperm quality parameters for better
understanding the role of this enzyme in male fertility.
Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved.
Ejaculate samples were collected using an artificial vagina, in October (the breeding
season) and April (the non-breeding season). Blood samples were collected prior to
sperm collection. Sperm volume (ml), motility (%), mass activity (1-5),
concentration (×106), viability (%), abnormal acrosome morphology (%) and
abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and
erythrocyte G6PD were determined and the relation of sperm parameters with G6PD
activity was evaluated.
Results: Erythrocyte G6PD activity was higher (p≤0.001), whereas spermatozoa
G6PD activity was lower (p≤0.001) in the breeding season (1.928±0.231 U/g
hemoglobin, 129.65±28.41 U/g protein, respectively) from that in the non-breeding
(0.530±0.066 U/g hemoglobin, 562.36±94.92 U/g protein, respectively). There were
also significant differences among sperm quality parameters within the seasons.
Positive correlation was determined between spermatozoa G6PD activity (r=0.053,
p=0.03 and sperm concentration in the breeding season.
Conclusion: Higher spermatozoa G6PD activity in October, where the level of
polyunsaturated fatty acids is suggested to be increased, may reflect the increased
need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity
for the oxidative balance.
Spermatozoa; Erythrocyte; Ram; Glucose-6-phosphate dehydrogenase.