International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 16, No. 5, 2018, pp. 335-340
Bioline Code: rm18037
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 16, No. 5, 2018, pp. 335-340
© Copyright 2018 - International Journal of Reproductive BioMedicine
In vitro antiapoptotic effects of the calligonum extract on spermatogonial stem cells|
Barati, Shirin; Movahedin, Mansoureh & Batooli, Hossien
Background: Spermatogonial stem cells are the foundation of spermatogenesis and
male fertility. So, their maintenance and culture are very important.
Objective: In this study, we assessed protective effects of the Calligonum on in
vitro viability and apoptotic and antiapoptotic genes expression of spermatogonial
Materials and Methods: After 24 hr of culture, the spermatogonial stem cells were
treated with 30 μM dose of H2O2 and then 10 μg/ml the Calligonum extract was
added for 3 wks. Viability was assessed by Trypan blue, apoptosis using PI-Annexin
and finally Bax, Bcl-2 and P53 genes expression by Real-Time Polymerase chain
Results: After 3 wk of treatment, viability in the Calligonum extract+H2O2 group
was significantly higher than H2O2 group alone (p=0.001). In the Calligonum
extract+H2O2 group, apoptosis, as well as expression of apoptotic genes (Bax and
P53), was significantly lower than the group treated with H2O2 alone.
Conclusion: The results of this study showed that 30 μM H2O2 increased apoptosis
but decreased viability in spermatogonial stem cells. Calligonum has antioxidant
properties that can reduce apoptosis, Bax and P53 expression and increase the
viability and Bcl-2 expression.
Stem cell; Apoptosis; Calligonum; Viability.
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