International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 16, No. 5, 2018, pp. 305-314
Bioline Code: rm18034
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 16, No. 5, 2018, pp. 305-314
© Copyright 2018 - International Journal of Reproductive BioMedicine
Effect of the estrus cycle stage on the establishment of murine endometriosis lesions|
Kiani, Kiandokht; Movahedin, Mansoureh; Malekafzali, Hossein; Mirfasihi, Faramarz; Sadati, Seyedeh Nargess; Moini, Ashraf; Ostad, Seyed Nasser & Aflatoonian, Reza
Background: Establishment of a standardized animal endometriosis model is
necessary for evaluation of new drug effects and for explaining different ethological
aspects of this disease. For this purpose, we need a model which has more similarity
to human endometriosis.
Objective: Our objective was to establish an autologous endometriosis mouse
model based on endogenous estrogen level and analyze the influence of estrus cycle
on the maintenance of endometriotic lesions.
Materials and Methods: In this experimental study, endometriotic lesions were
induced in 52 female NMRI mice by suturing uterine tissue samples to the
abdominal wall. The transplantation was either performed at proestrus/estrus or at
metestrus/diestrus cycles. Urine-soaked beddings from males and also male
vasectomized mice were transferred to the cages to synchronize and maintenance of
estrus cycle in female mice. The mice were sacrificed after different transplantation
periods (2, 4, 6 or 8 wk). The lesions size, macroscopic growth, model success rate,
histological and immune-histochemical analyses were assessed at the end.
Results: From a total of 200 tissue samples sutured into the peritoneal cavity, 83
endometriotic lesions were confirmed by histopathology (41.5%). Model success
rate for proestrus/estrus mice was 60.7% vs. 79.2% for metestrus/diestrus mice. The
endometriotic lesions had similar growth in both groups. Number of caspase-3,
Ki67-positive cells and CD31-positive micro vessels were also similar in
endometriotic lesions of two groups.
Conclusion: If we maintain the endogenous estrogen levels in mice, we can induce
endometriosis mouse model in both proestrus/estrus and metestrus/diestrus cycle
without any significant difference.
Endometriosis; Animal models; Transplantation; Autologous; Estrus cycle.
Alternative site location: http://www.ijrm.ir