Ovary stereological features and serum biochemical factors following induction of polycystic ovary syndrome with testosterone enanthate in mice: An experimental study|
Kalhori, Zahra; Mehranjani, Malek Soleimani; Azadbakht, Mehri & Shariaatzadeh, Mohammad Ali
Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder featured
by insulin resistance and hyperandrogenism. Testosterone enanthate can induce
PCOS in mice models.
Objective: We investigated the ovary stereological features along with the oxidative
stress and inflammatory factors in mice following PCOS induction using
Materials and Methods: Twelve female NMRI mice (3 wk old) were divided into 2
groups (n=6/each): Control and PCOS. PCOS was induced through daily injections
of testosterone enanthate (1 mg/100g subcutaneous s.c for 5 wk). Finally, ovaries
were studied stereologically. The serum levels of the follicle-stimulating hormone,
luteinizing hormone, testosterone, interleukin-6, and tumor necrosis factor-α were
measured using ELISA kit. Serum levels of Malondialdehyde and the antioxidant
capacity were measured relatively using thiobarbituric acid and ferric reducing
antioxidant power assay.
Results: The mean total volume of ovary and the mean volume of cortex (p<0.001),
volume of oocyte in the preantral (p=0.011) and antral follicle (p=0.015), thickness
of zona pellucida (p=0.016), the number of antral follicles (p=0.012), the serum
levels of follicle-stimulating hormone (p<0.001) and the antioxidant capacity
(p=0.020) reduced significantly in the PCOS group compared to the control. The
number of primary (p=0.017) and preantral (p=0.006) follicles and the serum levels
of testosterone (p<0.001), Luteinizing hormone (p=0.002), Malondialdehyde,
Interleukin 6 and Tumor necrosis factor-α (p<0.001) showed a significant increase
in the PCOS group compared to the control.
Conclusion: Testosterone enanthate induced PCOS causes stereological features in
the ovary, increases the oxidative stress and inflammatory markers in mice.
Polycystic ovary syndrome; Inflammation; Oxidative stress; Mice.