Protective effect of cerium oxide nanoparticle on sperm quality and oxidative damage in malathion-induced testicular toxicity in rats: An experimental study|
Moridi, Heresh; Hosseini, Seyed Abdolhakim; Shateri, Hossein; Kheiripour, Nejat; Kaki, Arastoo; Hatami, Mahdi & Ranjbar, Akram
Background: Malathion is an organophosphorus pesticide that commonly used in
many agricultural and non-agricultural processes. Previous studies have reported the
effects of melatonin on the reproductive system. Cerium dioxide nanoparticles
(CeNPs) due to their antioxidative properties are promising to impact on the
development of male infertility.
Objective: The aim of this study was to evaluate the effect of CeNPs on oxidative
stress and sperm parameters after malathion exposure of male rats.
Materials and Methods: 36 adult male Wistar rats were divided into 6 groups
(n=6/each): Control, CeNPs -treated control (15 and 30 mg/kg/day), malathion (100
mg/ kg/day), and CeNPs -treated malathion groups (15 and 30 mg/ kg/day). At the
end of the study (4 wk), the sperm counts, motility, and viability in the testis of rats
were measured, also lipid peroxidation, total antioxidant capacity, and total thiol
groups in homogenate testis were investigated.
Results: Malathion significantly reduced sperm count, viability, and motility than
the control rats (p<0.001). Co-treatment of malathion with CeNPs 30 mg/kg had a
protective effect on sperm counts (p=0.03), motility (p=0.01), and viability
(p<0.001) compare to malathion group. Also, the results showed that malathion
reduced testis total anti-oxidant capacity, the total thiol group, and increased testis
malondialdehyde than the control rats (p<0.001). CeNPs 30 mg/kg are increased
total antioxidant capacity (p<0.001) and total thiol group (p=0.03) compared to
malathion group. CeNPs at both doses (15 and 30 mg/kg) improved
malondialdehyde than the malathion group (p<0.001 and p=0.01 respectively).
Conclusion: CeNPs 30 mg/kg administered considerably restored testicular changes
induced by malathion. The improvement of oxidative stress by CeNPs may be
associated with increased sperm counts, motility and viability in the testis.
Testis; Malathion; Oxidative stress; Nanoparticles; Rat.