International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 16, No. 3, 2018, pp. 149-158
Bioline Code: rm18018
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 16, No. 3, 2018, pp. 149-158
© Copyright 2018 - International Journal of Reproductive BioMedicine
The effect of resveratrol on oxidative stress in the liver and serum of a rat model of polycystic ovary syndrome: An experimental study|
Ghowsi, Mahnaz; Khazali, Homayoun & Sisakhtnezhad, Sajjad
Background: Studies of oxidative status in polycystic ovarian syndrome (PCOS)
patients are limited with inconsistent results. The effects of resveratrol as a natural
antioxidant on oxidative status in PCOS aren’t clear.
Objective: This study evaluated effects of resveratrol on oxidative stress in the liver
and serum of the PCOS rats.
Materials and Methods: Fifteen female Wistar rats (3 wk old) were divided into 3
groups (n=5/each e): Control group, PCO-Control group, and PCO-Resveratrol
group. For induction of polycystic ovary phenotype, testosterone enanthate 10
mg/kg was injected for 35 days subcutaneously. Then, resveratrol 10 mg/kg was
injected intraperitoneally for 28 days to rats of the PCO-Resveratrol group. Ovarian
sections were stained with hematoxylin/eosin. The serum glucose and insulin and
the levels of malondialdehyde (MDA) and total antioxidant capacity (TAC) in serum
and liver were measured.
Results: Control animals showed normal ovarian morphology and PCO-Control
animals exhibited cystic follicles. There were no significant differences in liver TAC
between groups. The serum MDA (p=0.034), and homeostatic model assessment
insulin resistance (HOMA-IR) (p=0.014) levels in PCO-Control rats were higher
than the controls. The liver MDA in PCO-Control rats was more than that of
controls (p=0.001). The HOMA-IR (p=0.008) and serum MDA (p=0.006) levels in
PCO-Control rats were more than those of PCO-Resveratrol rats (p=0.008). In PCOResveratrol
group, serum TAC was higher than that of PCO-Control group
(p=0.022) and liver MDA was more than controls (p=0.01).
Conclusion: Results indicated that the induction of PCOS in rats increased lipid
peroxidation and insulin resistance and resveratrol improved these complications.
Oxidative stress; Polycystic ovary syndrome; Resveratrol; Liver.
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