International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 16, No. 2, 2018, pp. 83-92
Bioline Code: rm18011
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 16, No. 2, 2018, pp. 83-92
© Copyright 2018 - International Journal of Reproductive BioMedicine
Endometrial mesenchymal stem stromal cells in mature and immature sheep: An in vitro study|
Ghobadi, Farnaz; Rahmanifar, Farhad; Mehrabani, Davood; Tamadon, Amin; Dianatpour, Mehdi; Zare, Shahrokh & Jahromi, Iman Razeghian
Background: Endometrial mesenchymal stem stromal cells (EnMSCs) are critical
for uterine function, repair, and regeneration.
Objective: This study introduced isolation technique of EnMSCs and compared the
characteristics of EnMSCs in mature and immature ewes.
Materials and Methods: Endometrial tissue samples from the uterus of 10 ewes
were collected from the slaughterhouse. Endometrial cells were isolated from tissue
using cold incubation and then chopping and treating was performed with
collagenase type I. Isolated cells were cultured in cell culture medium and then
attached cells to flasks were harvested as EnMSCs and subcultured. To enumerate
the cells, the population doubling time (PDT) was determined and 2.2×104 cells in
passage 4 were seeded into 24-well culture plates to compare the growth curves of
isolated cells. Reverse transcription polymerase chain reaction (RT-PCR) was
performed for detection of CD34 and CD73 markers. The osteogenic and adipogenic
potential of isolated cells were determined using differentiation tests.
Results: EnMSCs adhered to the flasks and displayed spindle-shape. Based on
findings of the cell count and the growth curves, the EnMSCs growth was
significantly more prominent in immature ewes in comparison to mature sheep. The
PDT of EnMSCs in immature ewes was about 21 hr whereas this time period was
two times higher (45 hr) in mature sheep. RT-PCR analyses of EnMSCs were
positive for CD73 and negative for CD34. EnMSCs were differentiated into
osteoblasts and adipocytes.
Conclusion: Based on mesenchymal stem cells characters confirmed in EnMSCs,
they can be a candidate for cell therapy and regenerative medicine.
Endometrium; Mesenchymal stromal stem cells; Characterization; Differentiation; Sheep.
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