Evaluation of changes in the expression of Wnt/β-catenin target genes in mouse reproductive tissues during estrous cycle: An experimental study|
Zavareh, Saeed; Gholizadeh, Zahara & Lashkarbolouki, Taghi
Background: The Wingless-type (Wnt)/β-catenin signaling pathway controls cell
homeostasis. Reproductive tissues are dynamic in response to steroidal hormone
changes. Steroidal hormones are known to control the Wnt/β-catenin pathway, but
their role in reproductive tissues remains unknown.
Objective: The present study aims to investigate the expression patterns of Wnt/β-catenin target genes in mouse reproductive tissues during the normal estrous cycle.
Materials and Methods: In this experimental study, 16 adult NMRI mice were
grouped as proestrus, estrus, metestrus, and diestrus according to vaginal smear and
histological evaluation of uterine and ovarian tissues. Uterine horns and ovarian
tissues were collected. Reverse transcription quantitative polymerase chain reaction
was performed to evaluate the expression of Wnt/β-catenin target genes (Myc2,
Ppard, Id2, Birc5, and Ascl2) at different stages of the estrous cycle.
Results: The expression levels of Id2, Ascl2, and Pprd in uterine tissue were
significantly higher at the proestrus phase than at the other stages. Meanwhile, Birc5
expression in uterine tissue was significantly higher at the metestrus stage than at the
other stages. Furthermore, Myc2 expression was significantly higher at the diestrus
stage than at the estrus and metestrus stages. In the ovarian tissue, the highest
expression of Id2, Ascl2, and Birc5 was detected at the proestrus stage, whereas the
highest expression of Myc2 and Ppard was observed at the estrus stage.
Conclusion: This study showed that Wnt/β-catenin target genes profiles are
different among estrous cycle. It seems that different hormonal profiles during
estrous cycles play a key role in the expression pattern of Wnt/β-catenin target genes
in ovarian and uterine tissue.
Wnt signaling pathway; Beta catenin; Estrous cycle; Mice.