Resveratrol supplementation rescues pool of growing follicles and ovarian stroma from Cisplatin-induced toxicity on the ovary in Sprague-Dawley rats: An experimental study|
Chinwe, Gbotolorun Stella; Azuka, Okafor Izuchukwu & Adaeze, Ndoeche Chidinma
Background: Cisplatin is a potent antineoplastic agent for many cancers but causes
several levels of gonadal damage. Ovarian toxicity is a major concern of young
cancer patients undergoing chemotherapy.
Objective: This study sought to examine the effect of Cisplatin and Resveratrol
supplementation on ovarian function in Sprague-Dawley rats.
Materials and Methods: In this experimental study, 45 cyclic Sprague-Dawley rats
with an average weight of 160 gr were divided into 9 groups (n=5/group). Group 1
was used as control and received distilled water. Groups 2 and 9 received Cisplatin
only. Groups 3, 4, and 5 received different doses of Resveratrol after a single dose of
Cisplatin. Groups 6, 7, and 8 received Resveratrol before Cisplatin. At sacrifice, the
ovary was analyzed for histopathology, biochemical indices of oxidation and
Results: Relative and absolute organ weights were notably increased (p=0.001,
0.01) in the prophylactic groups relative to the groups that received Resveratrol after
Cisplatin. Also, glutathione, superoxide dismutase and catalase were significantly
increased (p=0.047, 0.01, 0.023) in a dose-dependent manner when compared to
Cisplatin group only. Malondialdehyde decreased significantly (p=0.001) in the
groups that received high dose Resveratrol compared with the control and Cisplatin
alone groups. Although oestrogen showed no significant difference within the
groups (p=0.48), Resveratrol significantly increased progesterone, follicle
stimulating hormone and luteinizing hormone levels (p=0.007, 0.001, 0.006) at high
doses when compared with Cisplatin alone groups. Ovarian histoarchitecture was
best preserved in the prophylactic groups in a dose-dependent manner.
Conclusion: Resveratrol supplementation confers protection and preserves ovarian
follicles from Cisplatin toxicity in Sprague-Dawley rats.
Cisplatin; Ovary; Resveratrol; Sprague-Dawley rat.