International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 15, No. 12, 2017, pp. 795-802
Bioline Code: rm17093
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 15, No. 12, 2017, pp. 795-802
© Copyright 2017 - International Journal of Reproductive BioMedicine
Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study|
Jabarpour, Masoome & Tajik, Parviz
Background: Spermatogonial stem cells (SSCs) are undifferentiated cells which are
highly reproducible and expandable. Several studies have been conducted to
reproduce these cells in culture. They used growth factors, hormones and different
feeder cells to improve survival and proliferation of SSCs.
Objective: This study was conducted to evaluate the effects of follicular stimulating
hormone (FSH) on gene expression of fibroblast growth factor (FGF2) and glial
cell-derived neurotrophic factor (GDNF) in Sertoli cells.
Materials and Methods: Sertoli cells and SSCs were isolated from 3-5 month-old
calves. Bovine testicular cells were cultured for 15 days with or without FSH.
Identification of these cells was confirmed by immunocytochemistry analysis.
Colony formation of SSCs was evaluated using an inverted microscope. The gene
expression of FGF2 and GDNF and the gene markers bcl6b, thy-1, and C-kit were
evaluated using the quantitative RT-PCR technique.
Results: The results indicated that FSH increased colonization of SSCs. the
expression of GDNF, FGF2, and markers of undifferentiated spermatogonia was
increased following culture in control and FSH groups (p<0.05), this increase was
more in FSH group. Conversely, the expression of C-kit was decreased in both
Conclusion: The results showed that FSH can increase the self-renewal of SSCs in
vitro via upregulation of GDNF and FGF2 expression in Sertoli cells.
Sertoli cell; SSCs; FSH; Self-renewal.
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