International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 15, No. 11, 2017, pp. 713-718
Bioline Code: rm17083
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 15, No. 11, 2017, pp. 713-718
© Copyright 2017 - International Journal of Reproductive BioMedicine
Comparison of Chlamydia trachomatis infection among infertile and fertile women in Ahvaz, Iran: A case-control study|
Joolayi, Fatemeh; Navidifar, Tahereh; Jaafari, Razieh Mohammad & Amin, Mansour
Background: Chlamydia trachomatis (C. trachomatis) is the main cause of
bacterial sexually transmitted infections. In women, this infection can lead to tubal
Objective: In this study we investigated C. trachomatis among infertile and fertile
women with both polymerase chain reaction (PCR) and ELISA methods in Ahvaz,
Materials and Methods: This case-control study was conducted at the Infertility
Clinic of University Jahad, Ahvaz, Iran from January to August 2017. A total of 225
vaginal swabs and blood samples (100 infertile and 125 fertile women) were
collected. Detection of C. trachomatis DNA was performed from vaginal swabs by
amplification of MOMP gene. Also, anti C. trachomatis immunoglobulin M (IgM)
and immunoglobulin G antibodies in the serum samples were recognized by
enzyme-linked immunosorbent assay (ELISA).
Results: Results showed that, 6 (6%) infertile and 2 (1.6%) fertile women were
positive for IgM (p=0.21). Also, PCR was positive for C. trachomatis infection in 5
infertile (5%) and 2 fertile women (1.6%) (p=0.35). We did not find any seropositive
immunoglobulin G in both groups.
Conclusion: In this study, no significant difference was found between fertile and
infertile groups for C. trachomatis infection. Also, the correlation between IgM and
PCR results revealed a relatively strong agreement and seems both PCR and IgM
assays are appropriate for the accurate diagnosis of C. trachomatis infections.
C. Trachomatis; Infertility; PCR; IgM; IgG.
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