International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 15, No. 10, 2017, pp. 661-668
Bioline Code: rm17078
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 15, No. 10, 2017, pp. 661-668
© Copyright 2017 - International Journal of Reproductive BioMedicine
Effect of Citrullus colocynthis hydro-alcoholic extract on hormonal and folliculogenesis process in estradiol valerate-induced PCOs rats model: An experimental study|
Barzegar, Mohammad Hossein; Khazali, Homayoun; Kalantar, Seyyed Mehdi & Khoradmehr, Arezoo
Background: Citrullus colocynthis (CCT) is used as the anti-diabetic and
antioxidant agent. Polycystic ovarian syndrome (PCOS) is a reproductive disorder
which level of gonadotropins and sexual hormones are imbalanced.
Objective: We evaluated the effect of CCT hydro-alcoholic extract on hormonal and
folliculogenesis process in estradiol valerate-induced PCOs rats’ model.
Materials and Methods: 40 female adult Wistar rats divided into five groups
(n=8each: Group I (control) only injected by sesame oil as estradiol valerate solvent,
group II (Sham) was orally received normal saline after estradiol valerate- induced
polycystic ovarian syndrome (4 mg/rat estradiol valerate, intramuscularly), and three
experimental groups, that after induction of PCOS within 60 days, received orally 50
mg/kg CCT extract (group III), 50mg/kg metformin (group IV), and CCT extract+
metformin (group V) for 20 days. The serum concentration level of luteinizing,
testosterone and follicle stimulating hormones were measured using ELISA method
and the serum concentration level of glucose were measured using the oxidative
method (glucose meter). Histological study of ovary tissue carried out by
Results: There was a significant reduction in luteinizing hormone and testosterone
in III-V groups compared to Sham group, whereas follicle stimulating hormone in
III-V groups was not significantly changed in comparison with Sham group.
Histological investigations showed a significant increase in number of preantral and
antral follicles and corpus luteum in the experimental groups compared to group II.
Conclusion: Marked improvement in hormonal and histological symptoms of PCOS
may be due to CCT effects hence, CCT can potentially be considered as an effective
drug for treatment of PCOS.
Polycystic ovary syndrome; Gonadotropins hormones; Follicugenesis; Rat Citrullus colocynthis.
Alternative site location: http://www.ijrm.ir