The effects of Salvia officinalis L. on granulosa cells and in vitro maturation of oocytes in mice|
Monsefi, Malihezaman; Nadi, Akram & Alinejad, Zeinab
Background: Salvia officinalis L. has been used since ancient times but there are
little data about effects of this herb on normal reproductive cells.
Objective: To investigate the toxicity effects of Salvia officinalis L. on granulosa
cells (GCs) and maturation of oocytes.
Materials and Methods: GCs and oocytes were extracted from superovulated
ovaries of immature mice. The cells were treated with concentrations of 10, 50, 100,
500, and 1000 μg/ml of Salvia officinalis hydroalcoholic extracts and compared with
the control culture. Bioviability, chromatin condensation, estradiol and progesterone
concentrations, lipid synthesis, apoptosis, and alkaline phosphatase activity of GCs
were measured. In vitro maturation of oocytes by determination of different
maturation stages of oocytes including germinal vesicle, germinal vesicle breaks
down, and metaphase II were examined.
Results: The results revealed that 500 and 1000 μg/ml concentrations of Salvia
officinalis L. were toxic. The most of the GCs were in the early stages of apoptosis
in 100 μg/ml treated culture and cell death happened with 500 μg/ml treatment.
Progesterone concentration was reduced in 100 μg/ml and higher doses but estradiol
concentration and alkaline phosphatase showed opposite effects. The lipid droplets
content of GCs reduced significantly in all groups especially in 500 and 1000 μg/ml.
Finally, oocyte’s nucleus and cytoplasm showed a high level of condensation, and
meiosis rate reduced in all treated cultures.
Conclusion: Our findings suggested that higher dose of Salvia officinalis
hydroalcoholic extracts inhibits, oocyte maturation, GCs bioviability, proliferation,
Granulosa cell; In vitro maturation; Oocytes; Ovary; Salvia officinalis.