International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 15, No. 8, 2017, pp. 471-478
Bioline Code: rm17059
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 15, No. 8, 2017, pp. 471-478
© Copyright 2017 - International Journal of Reproductive BioMedicine
Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty|
Sistani, Maryam Nezhad; Maleki, Anahid; Salimi, Maryam; Novin, Marefat Ghaffari & Nazarian, Hamid
Background: common use of sevoflurane in congenital defects during repeated
surgeries may have detrimental effects on spermatogenesis after puberty.
Objective: This study investigated sevoflurane effects on spermatogenesis process
in male mature mice after exposure in prepubertal time.
Materials and Methods: 24 neonatal NMRI male mice were randomly classified in
three groups. Experimental 1 and 2 groups (exposure to 1 minimum alveolar
concentration (MAC) and 2 MAC sevoflurane, respectively in 2 lit/min oxygen (O2)
for 7 days (30 min, daily) and control. All groups were sacrificed after 2 months.
Histological assessment, immunohistochemistry and apoptosis process was done.
Bax and Bcl2 expression was evaluated in the testicular tissue by real time Poly
Results: Our results showed that the integrity of testicular tissue was preserved in
both experimental groups. Count of spermatogonial cells had significant decrease in
group 2 compared to others. The rate of apoptosis in spermatogonial cells was
15±3% and 9±2% in the group 2 and 1, respectively. Also, Bax/Bcl2 ratio was
0.2615, 1.0070 and 9.3657 in control, experimental group 1 and 2, respectively. This
result was significant (p≤0.002) between groups 2 with other groups.
Conclusion: Continuous exposure of 2 MAC sevoflurane in 2 lit/min O2
simultaneous during prepubertal may create more testicular tissue damage in terms
of cellular and molecular function compared to continuous exposure to lower level
of sevoflurane by increase in ratio of Bax/Bcl2 and apoptosis in germ cells after
Spermatogonial cell; Prepubertal; Germ cells.
Alternative site location: http://www.ijrm.ir