In vitro development of embryos from experimentally Kerack-addicted Mice|
Mohammadzadeh, Elham; Amjadi, Fatemeh-Sadat; Movahedin, Mansoureh; Zandieh, Zahra; Nazmara, Zohreh; Eslahi, Neda; Shirinbayan, Peymaneh; Asgari, Hamid Reza; Azad, Nahid; Salimi, Maryam & Koruji, Morteza
Background: Prenatal drug exposure, as a common public health concern, is
associated with an increased risk of adverse effects on early embryo development.
Objective: To investigate the in vitro development of - embryo from experimentally
Materials and Methods: Twenty-five female mice were studied in five groups:
control, vehicle, and three experimental groups of Kerack-dependent mice (I, II, and
III) which received different doses of Kerack for 14 days. After the establishment of
addiction model (7 days), experimental groups I, II, and III were given Kerack
intraperitoneally at the doses of 5, 35, and 70 mg/kg, twice a day for a period of 7
days, respectively. The vehicle group received normal saline and lemon juice whilst
the control group just received water and food. Morulae were obtained through
oviduct flashing. The survived embryos were cultured in T6+ 5mg/ml bovine serum
albumin. The developmental rates up to hatched stage daily and embryo quality
(differential staining and Tunnel staining) were also assessed
Results: The developmental potential of embryos obtained from the addicted mother
was significantly decreased in comparison with control group. There was a
significant reduction in the rate of blastocyst formation in the high dose Kerack
dependent group. However, in addicted mice there was reduction in the total cell
number (40.92% vs. 65.08% in control) and, inner cell mass percentage (17.17% vs.
26.15% in control) while apoptotic cells numbers were increased (7.17 vs. 1.46 in
Conclusion: The Kerack addiction during pregnancy retards preimplantation
development and induces apoptosis.
Addiction; Preimplantation; Embryo development; Apoptosis; Mouse.