Combined protective effect of zinc oxide nanoparticles and melatonin on cyclophosphamide-induced toxicity in testicular histology and sperm parameters in adult Wistar rats|
Torabi, Fereshte; Shafaroudi, Majid Malekzadeh & Rezaei, Nourollah
Background: Cyclophosphamide (CP) has been known as an anticancer drug with
several side effects on various organs such as a male reproductive system that can
Objective: To evaluate the possible combined effects of zinc oxide nanoparticles
(nZno) and melatonin (Mel) on sperm parameters and histopathological changes of
the testis in CP-treated rats.
Materials and Methods: 42 adult male Wistar rats were divided into six groups.
GI: control, GII: 60 mg/kg/wk CP, GIII and GIV, 10 mg/kg/wk Mel and 5mg/kg/wk
nZno and GV: 5 mg/kg/wk nZno and 10 mg/kg/wk Mel were given 2 hr prior to CP
injection, respectively,GVI: 5mg/kg/wk nZno and 10 mg/kg/wk Mel simultaneously.
After 8 wk of treatment, rats were sacrificed and testis and epididymis were
harvested for further evaluation.
Results: The CP-treated group showed significant decreases in the body, testes and
epididymis weights and sperm parameters (sperm count, viability, motility) with an
increase abnormal sperms when compared with the control (p<0.001), as well as
many histological alterations included decreased diameters of seminiferous tubules
and Johnsen’s Testicular Score (with degeneration, desquamation, multi-nucleated
giant cell formation), whereas combined treatment (GV), showed more protective
effects on CP-induced reproductive system damage compared with groups III or IV
Conclusion: These results suggest simultaneous administration of Mel and nZno
have more effectively protections against CP-induced reproductive damage than Mel
or nZno alone.
Cyclophosphamide; Zinc oxid; Melatonin; Toxicity; Sperm.