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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 11, No. 11, 2013, pp. 875-882
Bioline Code: rm13111
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Reproductive BioMedicine, Vol. 11, No. 11, 2013, pp. 875-882

 en Effect of oocyte activation with calcium ionophore on ICSI outcomes in teratospermia: A randomized clinical trial
Eftekhar, Maryam; Janati, Sima; Rahsepar, Mozhgan & Aflatoonian, Abbas


Background: Chemical activation is the most frequently used method for artificial oocyte activation (AOA), results in high fertilization rates.
Objective: This prospective, randomized, unblinded clinical study aimed to evaluate the efficiency of calcium ionophore on fertilization and pregnancy rate after intracytoplasmic sperm injection (ICSI) in infertile men suffer from teratoospermia.
Materials and Methods: Thirty eight women with teratoospermic partner underwent ICSI with GnRH-antagonist protocol. A total of 313 metaphase II (MII) oocytes were randomly divided after ICSI into two groups: In the oocytes of the control group (n=145), routine ICSI was applied. Oocytes in the AOA group (n=168) immediately after ICSI, were cultured in culture medium supplemented with 5 μΜ calcium/100 μM calcium ionophore (A23187) for 5 minutes and then washed at least three times with MOPS solution. In both groups, the fertilization was evaluated 16-18 hours after ICSI.
Results: The number of embryos obtained and fertilized oocytes were significantly different between two groups (p=0.04). There was no significant difference between the two studied groups regarding the fertilization and cleavage rate (95.33% vs. 89.56 %; and 84.4% vs. 87.74%; respectively, p=0.06). Implantation rate was higher in AOA group than in control group, but the difference was not significant. (17.64% vs. 7.4%, p=0.14). No significant differences were observed in chemical and clinical pregnancy rate between groups (47.1% vs. 16.7%, p=0.07; and 41.2% vs. 16.7%; p=0.14, respectively).
Conclusion: Oocyte activation with calcium ionophore may improve ICSI outcomes in infertile men suffer from teratoospermia. Although, we found no significant difference in the implantation and pregnancy rate between the two groups of patients. Further study with more cases can provide greater value.

Intracytoplasmic sperm injection; Calcium ionophore; Oocyte activation; Fertilization

© Iranian Journal of Reproductive Medicine
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