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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 11, No. 9, 2013, pp. 747-752
Bioline Code: rm13094
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Reproductive BioMedicine, Vol. 11, No. 9, 2013, pp. 747-752

 en The effects of ex vivo cold-storage on cryopreservation of the goat ( Caprus hircus check for this species in other resources ) epididymal sperm
Hoseinzadeh-Sani, Seyed Kamal-Aldin; Barati, Farid & Mahabady, Mahmoud Khaksary


Background: There are many studies focused on long or short storage time of epididymal sperm (EPS) of different species. There are limited studies on preservation or cryopreservation of the domestic goat EPS.
Objective: The aim of the present study was to evaluate the effects of ex vivo cold-storage on freezing of EPS from goat ( Caprus hircus check for this species in other resources ).
Materials and Methods: In a split-plot design the caprine testes-epididymides (40 pairs) were divided to 4 storage-time groups equally (0, 24, 48 and 72 h), then subjected to cryopreservation using Bioxell. Sperm parameters were analyzed before and after freezing.
Results: Duration of cold-storage as well as freezing at all storage-time points reduced sperm viability and progressive motility while increased sperm tail abnormalities (p<0.0001). Freezing reduced the percentage of cytoplasmic droplets (p<0.0001). The percentage of detached heads was increased at all storage-time points following freezing (p=0.0019), except at time 0 h.
Conclusion: It can be concluded that cold storage [in refrigerator (4oC) for 72 h] of epididymides efficiently protected the goat EPS in terms of progressive motility and viability. However, cold-storage may not protect the goat EPS against cryopreservation with Bioxell.

Epididymal sperm retrieval; Cryopreservation; Refrigeration; Cold-storage; Goat

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