International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 10, No. 5, 2012, pp. 409-412
Bioline Code: rm12057
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 10, No. 5, 2012, pp. 409-412
© Copyright 2012 - Iranian Journal of Reproductive Medicine
The effect of various concentrations of myo-inositol in culture medium on development of bovine embryos|
Soltani, Leila; Kafilzadeh, Farokh & Shabankareh, Hamed Karami
Background: Progress achieved in culture media formulations have resulted led to
an improvement in maintaining the mammalian embryo in culture throughout the
preimplantation and pre-attachment period.
Objective: The objective of this study was to evaluate the effect of various
concentrations of myo-inositol during in vitro fertilization of bovine oocytes on
subsequent embryo development.
Materials and Methods: Bovine cumulus oocytes complexes (COCs) were matured
in vitro at 39°C, in humidified 5% CO2 atmosphere for 22-24 h. The COCs were coincubated
with epididymal spermatozoa of post mortem bulls in modified TALP
medium for 22-24hr. The fertilization medium used was: 1) TALP medium without
myo-inositol (control); 2) control+0.02 g/l myo-inositol; 3) control+0.03 g/l myoinositol;
4) control+0.04 g/l myo-inositol. Zygotes were cultured in vitro for 8 days
when the ratios of in vitro embryo development of the hatched blastocysts were
assessed and compared with the control group (p<0.05).
Results: The presence of 0.04 g/l myo-inpsitol significantly improved overall
morula and blastocyst rates (46.94%) compared to control (32.19%), but there was
no difference in the percentage of embryos successfully developed to the morula and
blastocyst stage when different levels of myo-inositol were used (46.94, 36.36 and
37.33% respectively). The mean percentage of cleavage rate was not significantly
affected by treatments.
Conclusion: These results suggest that, addition of 0.04 g/l myo-inositol in TALP
medium is more beneficial for subsequent bovine embryonic development.
Bovine, Myo-inositol, Fertilization, Embryo development.
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