International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 6, No. 2, 2008, pp. 71-76
Bioline Code: rm08012
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 6, No. 2, 2008, pp. 71-76
© Copyright 2008 - Iranian Journal of Reproductive Medicine
Adverse effects of ciprofloxacin on testis apoptosis and sperm parameters in rats|
Khaki, Arash; Heidari, Mahnaz; Novin, Marefat Ghaffari & Khaki, Amir Afshin
Ciprofloxacin is a commonly prescribed antibiotic in the treatment of
genitourinary tract infection.
The aim of this study was to investigate the effects of ciprofloxacin on testis apoptosis and sperm parameters in rat.
Materials and Methods:
Twenty male Wistar rats were selected and randomly divided
into two groups; control (n=10) and experimental (n=10). The experimental group was
orally received 12.5 mg/kg ciprofloxacin daily for 60 days and the control group just
received water and food. Rats were then killed and sperm removed from cauda
epididymis and analyzed for sperm motility, morphology, and viability. Testis tissues
were also removed and prepared for TUNEL assay to detect apoptosis.
Results showed that ciprofloxacin significantly decreased the sperm
concentration, motility (p<0.05) and viability (p<0.001). In addition, ciprofloxacin
treatment resulted in a significant decrease in the number of spermatogenic cells
(spermatogonia, spermatocyte, spermatid and sperm) in the seminiferous tubules when
compared with the control group. The apoptotic germ cells per seminiferous tubular
cross section was significantly increased in the experimental group (15.11±3.523) as
compared with the control group (7.3±0.762) (p<0.05).
It is concluded that ciprofloxacin has the toxicological effects on
reproductive system in male rats.
Ciprofloxacin, Testis, Sperm, Apoptosis, Rat.
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