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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 5, No. 3, 2007, pp. 121-126
Bioline Code: rm07026
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Reproductive BioMedicine, Vol. 5, No. 3, 2007, pp. 121-126

 en Comparative study of human papilloma virus DNA detection and results of histopathological examination of cervical colposcopic biopsy
Shalaby, Ahmad M. Ragab; Shalaby, Ahmad Mostafa & Naguib, Saed M.


Background: There is mounting evidence for HPV involvement in cervical cancer Human Papilloma Virus DNA is detected by hybridization techniques in 75 - 100% of patients with condylomas, precancerous cervical dysplasia, and invasive carcinoma.
Objective: The aim of this study was investigating factors that may contribute to false-negative colposcopic biopsy results in positive high-risk HPV DNA results.
Material and Methods: Patients positive for high-risk human papillomavirus (HPV) DNA with negative cervical histopathologic findings were examined between January 2004 and August 2006.
Results: Patients with atypical squamous cells of undetermined significance (ASC) in Papanicolaou smears, with positive HPV DNA results, but negative cervical histopathologic findings accounted for 4.5% of all ASC smears submitted for HPV DNA testing. We found 4% of the cases had focal HPV infection or mild dysplasia. When serial sectioning of the biopsy material were examined, we found that 29% had clinically significant lesions: HPV infection or cervical intraepithelial neoplasia CIN 1, 18%; CIN II/III, 8%; and dysplasia, not otherwise specified (which we can not categorize into any group), 3%. Of the remaining patients, follow-up revealed squamous abnormalities in 25%. About 5% of patients with positive HPV DNA results had a negative follow-up biopsy result. "False-negative" biopsies accounted for one third of cases.
Conclusion: In almost one third of cases, clinically significant lesions were found when additional levels were examined.

Cervix, Dysplasia, Hybrid,Human papilloma virus.

© Copyright 2007 - Iranian Journal of Reproductive Medicine
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