International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 5, No. 3, 2007, pp. 103-107
Bioline Code: rm07023
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 5, No. 3, 2007, pp. 103-107
© Copyright 2007 - Iranian Journal of Reproductive Medicine
In vitro application of Matrigel enhances human blastocyst formation and hatching|
Novin, Marefat Ghaffari; Heidari, Mahnaz; Akhondi, Mahdi A & Tehrani, Mahmood Jeddi
Background: Matrigel (extracellular matrix) can improve the growth of many cell types in vitro.
Objective: The aim of the present study was to determine the effect of Matrigel on the development of 2-4 cells human embryos in culture.
Material and Methods: Surplus 2-4 cells human embryos, resulting from ICSI, were divided into two groups (control and test). Quality of embryos in both groups was morphologically similar. The test group (n=140) was cultured in Hams' F10 supplemented with 10% human serum albumin and 150 µl liquid Matrigel. The control group (n=140) was cultured in the same medium devoid of Matrigel. Embryos were cultured for an additional 4 days and their morphology was assessed every 24 hours. Both groups were then statistically compared.
Results: The percentage of the human embryos that reached the morula stage in the control and test groups were 79.2% and 80%, respectively (p>0.05). However, 36.4% of embryos reached the blastocyst stage in the test group as compared to 5.7% in the control group after 144 hours in culture. This difference was statically significant (p <0.01). In addition, culture of embryos on Matrigel and medium versus medium alone significantly improved in vitro hatching (25.7% versus 3.5%; p <0.01).
Conclusion: Matrigel at low concentration enhances human blastocyst formation and hatching in vitro.
Matrigel, Extracellular matrix, Human embryos, Blastocyst, Hatching.
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